 # What is the total magnification of your microscope when using high power

what is the total magnification of your microscope when using high power 6. The microscope you are coloring and labeling is what type of microscope? _____ 10. scanning power 4X or 5X or 6X. So, the total magnification would be 400 X. (Do not look into the microscope yet. Resolving Power of Average Light Microscope: 0. The power of a microscope is described with a number followed by the letter "x". High power. Function :Doubling Total Magnification power (such as ,your microscope total magnification is 400X ,you use it, the magnification will increase 800X ). When using a high power microscope (also known as a compound microscope) it is best to start out with the lowest magnification, get your specimen in focus, and then move up to the higher magnifications one at a time. If the eyepiece is marked 10x and the objective 10x, then the total magnification is 100x. (Only use lens paper!) a) Calculate the low power magnification of this microscope. 65 = 650). The total magnification is equal to the magnification of the ocular multiplied by the magnification of the objective. The microscope will be your friend if you always use the following  The compound microscope is a useful tool for magnifying objects up to as much as 1000 times The eyepiece, also called the ocular lens, is a low power lens. Step 2: Click to lower power and focus again, recenter your specimen. At 100x magnification you will be able to see 2mm. 4x. Total magnification . 04mm (40µm). Draw the E exactly as it appears in your viewing field for each magnification. The total magnification of the image you see is the magnification of the ocular multiplied by the magnification of the objective you are using. Adjust the nosepiece so that the lowest magnification is in place. Thus, the total magnification possible with the microscope is the magnification achieved by the objective multiplied by high power? Support your answer. This might say 4X or 10X depending on the type of microscope that you are using. This activity works best with newsprint. In other words, total magnification using the LPO lens would be 40 times the original size, the MPO lens would achieve a total magnification of 100 times, while the HPO lens will Examine the microscope and familiarize yourself with the parts of the microscope. Germany, devised a high as 1. (Use medium power if your high power objective does not work. For total magnification multiply the ocular power (10x) times the objective lens that is in place. Each objective will have written the magnification. It will be a whole number greater than 1 and is usually followed by an “x”, as in 10x magnification. microscope. It’s one of those moments of frustration for students. Suppose you measure the low-power field of view with a ruler and it is 2 mm. power of the eyepiece (ocular lens - 10X) multiplied by the power of the objective lens. The magnifying power of an ocular lens varies from 5x to 30x, but normally 10X or 15X magnifying power is used. What is the total magnification produced when the high power objective is used? Introduction. The magnification of the ocular lenses on your scope is 10X. Oil Immersion Objective (100x) The same principle apply to stereo microscopes, a 10X eye piece combined with a 4X objective lens will produce 40X magnification. Magnification Your microscope has 3 magnifications: Scanning, Low and High. 1. Power Field Diameter (field of . This means that it magnifies an object 10X by itself  . Using the Compound Microscope Get a compound microscope from the cabinet, being sure to carry it upright and with two hands. b. You can see things many times larger than actual size. For example; if a stereo microscope with 10x eyepieces, the zoom knob is set to 5x and you also have a 0. 8. Most educational-quality microscopes have a 10x (10-power magnification) eyepiece and three objectives of 4x, 10x & 40x to provide magnification levels of 40x, 100x and 400x. Discussion: 1. The total magnification is the ocular x objective Calculating total magnification Practice problem: Calculate the low-power and the high-power total magnifications of a microscope with an eyepiece magnification of 10x, a low power objective of 20x, and a high-power objective of 40x. The total magnification is the ocular lens power times the objective power. The total magnification using the lenses can be determined by multiplying the objective lens with the ocular lens. e. Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. For example, if the maximum field diameter of the diaphragm is 20 millimeters, and the microscope has two lenses with a magnification each of 20x and 10x respectively, we can calculate the viewing field size as 20 millimeters divided by 200, equating to 0. To support the other components of the microscope 8 Coarse adjustment knob To move the body tube rapidly to allow focussing using the scanning and low power magnification. Apr 01, 2016 · Microscope Notes Parts of the Microscope DON’T use the coarse adjustment knob with medium or high power. Clean the microscope's ocular lens. 2. The microscope you are coloring and labeling is what type of microscope? _____ 10. 10X ( magnification = 10 multiplied by, for instance 4 for scanning power = 40 times normal size). Your microscope is referred to The total magnification viewed at any given time is a product of the eyepiece magnification times the objective magnification Example: when the 4X objective is locked in place, the total magnification is 10 x 4 = 40X. 2 with the naked eye. A microscope gives a total magnification of 1500X, but the image is too blurry to be useful. A microscope has an ocular objective of 10x and a high power objective of 50x, what is the microscope's total magnification? ___ 500x _____ You've reached the end of your free preview. a) Calculate the low power magnification of this microscope. Turn the revolving turret (2) so that the lowest power objective lens (eg. 4. Magnification. What is the total magnification of your microscope when using low power? ____ What is the total magnification of your microscope when using high power? ____ PART II: THE LETTER “e” (Practice) 1. Students should learn how to use and adjust the microscope from low power to high power to observe smaller and individual organisms. 2 mm. 4. Show your formula and all work. Mar 16, 2020 · High Power Objective Lens (40x) The high-powered objective lens (also called “high dry” lens) is ideal for observing fine details within a specimen sample. What magnifications are possible with your compound microscope? low power:_____ medium power:_____ high power:_____ 2. 4 mm X 1000 = 400 um c) diameter 2mm 1 cell = . The total magnification using the lenses can be determined by multiplying the objective lens with the ocular len Objective lenses. 3. Using your Microscope procedure knowledge and a plastic clear ruler, determine how many millimeters are in the field of view under 40X, 100X, 400X. Your microscope has 3 magnifications: Scanning (lowest power), Low and High. 75X - 7. ) _____ Match The Microscope Parts With Their Functions. The objectives are attached to a Using your newly acquired microscope skills, place the slide under the microscope on low power. Here's are the 100x Objective Lens of compound light microscope provides maximum total magnification of 100 . microscope has a power of 5x and you use an objective that is 10x, then the total magnification of the microscope at that time is 50x (5x10 = 50). It holds the objective lenses. The controls of the Total magnification = ocular power x objective A simple microscope or magnifying glass (lens) produces an image of the The distance from the back focal plane of the objective (not necessarily its the amount of image magnification is the objective magnifying power, which Fo Electron microscopes use elec-tron beams instead of light rays, and magnets This platform provides a surface for the placement of a slide with its specimen over magnification of the ocular lens, the total or overall linear magnifi In A Book. In addition to this, the ocular lens (eyepiece) has a magnification. They almost always consist of 4x, 10x, 40x and 100x powers. 5x auxiliary lens on it, the total magnification of that stereo microscope at the current setting is 10 x 2. Take a clear plastic ruler and examine the millimetre scale under low power. The total magnification of the microscope is calculated by multiplying the magnification of the objectives, with the magnification of the eyepiece. 0 mm X = 20 = . looking. It is what's required to provide the most Example. (If using a wet mount slide be sure there is no liquid on the outer surface of the coverslip or slide. low power? _____ b. Complete the table below by -power lens by the power of the low-power lens. Total magnification is calculated by multiplying the magnifi-cation of the objective by that of the eyepiece. For example, if you are looking through a stereo microscope that has 10x eyepieces, a zoom knob that is set to 2. What is the total magnification if the microscope is on low power (20X)? _____ 12. Calculate The Total Magnification Of An Object Viewed Under The Lowest Power Objective On Your Microscope. The field of view when using the 10x objective (100x total magnification) is 2 mm. After focusing the specimen under high power (400X or 430X, see above), rotate the high power objective out of the way and place a drop of immersion oil on the slide. In order to ascertain the total magnification when viewing an image with a compound light mic Light microscopes pass waves of visible radiation through lenses to increase the apparent size of the object being viewed. In addition to this, the ocular lens (eyepiece) has a magnification. will be wider, increasing the number of cells you are Microscopes Compound Microscopes. 7. What is the total magnification of your microscope when using high power? 400x Describe the position of the image of a letter e through the microscope compared to the position that it is placed on the slide Dec 08, 2020 · To calculate total magnification, find the magnification of both the eyepiece and the objective lenses. 5. Human Blood Smear (if time permits). If your microscope uses both an eyepiece and an objective lens, there is one step to do before you find the FOV. To calculate the total magnification achieved with a compound microscope, the magnification of the ocular lens is multiplied by the A compoun Solved example: magnifying power of compound microscope. Field Diameter or Field of View Magnification. 5. Record the Magnification of all power levels for your microscope in table 1. Use Table 1 to record your results as you calculate the total magnification obtained with each objective lens. 100x. What is the power of magnification when using the high power lens? If a microscope had a power of magnification of 200X, what would the power of the objective lens be if the eyepiece lens is 10X? List three possible combinations of eyepiece and objective lens powers if the power of magnification for a microscope was 500x? To calculate the magnification of a microscope, simply multiply the magnification of the microscope eyepiece by the magnification of the objective lens. 39 STAGE and STAGE CLIP – The stage is the flat surface upon which you will place each slide Oct 31, 2018 · High Power Objective Lens (40x) This is referred to as the high powered objective lens since it is ideal for observing the small details within a specimen sample. If 20 objects fit across the diameter of a low power field of view whose field diameter is 4000 μm, what would be the approximate size of each object? A microscope has an ocular objective of 10x and a high power objective of 50x what is the microscope's total magnification? The objectives are attached to what part of the microscope (it can be rotated to click lenses into the place?) 500 A microscope has an ocular objective of 10x and a high power objective of 50x what is the microscope's total magnification ? 9. Show your formula and all work. 2 mm to 2. Duration: Approximately 50 minutes is enough time to prepare, observe, and sketch onion cells. Check that your microscope is on LOW power. Typically, a compound microscope is used for viewing samples at high magnification (40 - 1000x), which is achieved by the combined effect of two sets of lenses: the ocular lens (in the eyepiece) and the objective lenses (close to the sample). (1 points) 3. 5. Under low power, the field of view is much larger and allows you to see much more of your specimen. the item should be Placed between the eyepiece tube and the eyepiece. 1 millimeter. Similarly, a low-power microscope doesn’t have a great enough magnification level for you to inspect bacteria successfully. 36 mm = 360 µm 5. D. ALWAYS leave the microscope with the low power objective in place and the body tube at its highest position. Total magnification of the microscope is found by multiplying the power of the ocular lens by the power of the objective lens. ) Calculate the low power magnification of this microscope. As a result, while your image will be magnified 1,000 times, it will yield no further useful information or finer resolution of detail. A typical lab-quality standard optical microscope will usually have four objective lenses, running from a low power of 4x to a high power of 100x. In this lab, you will not use the oil immersion lens; it is for viewing microorganisms and requires technical instructions not covered in this procedure. Label each with the total magnification and any cellular structures that can be identified. The reason for using a microscope is to magnify features to the point where new details can be resolved. ) Calculate the high power magnification of this microscope. 9. ruler and 50x, what is the total magnification of: (Show your math for full credit!) a. Describe The Proper Way To Carry A Microscope. Image 2 displays less of the room in the background but the DNA model is larger in appearance because the magnification is greater. distance. For example a magnification of 1000x can be achieved using a standard eyepiece magnification of 10x with a 100x objective lens. The magnification is standard, i. Example: You are looking at a slide through your LOW Power Lens. When coupled with a 10x (most common) eyepiece lens, total magnification is 40x (4x times 10x), 100x , 400x and 1000x. More magnification is NOT always better! Your microscope has 3 magnifications: Scanning, Low and High. With the l0X objective in use, the total magnification of the observed image is equal to the objective magnification (10X) times the ocular magnification (10X), or 100x. How to Use Microscope Immersion Oil Before using immersion oil, make sure that your 100x objective lens is made for use with immersion oil. Google Classroom Facebook Twitter. Magnification of objective lens x x x x. Total magnification: In a compound microscope the total magnification is the product of the objective and ocular lenses (see figure below). low power 10X or 12X. (Click Here To See Image) (Click Here To See Image) (Click Here To See Image) Total magnification of an object observed through the eyepieces (ocular lens) is calculated by multiplying the ocular lens magnification times the magnification of the objective lens being used (either: 4X, 10X, 20X, 40X, 100X, etc. ) Calculate the high power magnification of this microscope. Begin by focusing your sample using the 40x objective lens. Turn the microscope’s light on. compound . This problem has been solved! a) What is the highest magnification you could get using this microscope ?500x Ocular x high power = 10 x 50 = 500. Want to read the whole page? Apr 17, 2018 · Usually, the ocular lens has a magnification of 10x. What is the total magnification of your microscope when using low power? e. Magnifying power of an objective is determined by the dividing the optical tube length by the focal length of the lens. Most compound microscopes have a 10x eyepiece and three objectives, 4x, 10x and 40x. 1pc Biological Microscope Use 2X Microscope Barlow Eyepiece Magnification lens. Jan 25, 2021 · They have a magnification power of 40x-100X. b. We have gone over the use of your light microscopes during lab and your laboratory handout has Total magnification = Objective magnification X ocular magnification Magnification is not of much value unless resolving power is high When viewing a slide through the microscope make sure that the stage is all the eye with your hand) slowly move the stage upward using the coarse adjustment To get the total magnification take the power of the objective (4X, 10X, What is the total magnification of the microscope is you are using low power? Why is it important to make sure your specimen is in the center of your field of  16 Mar 2020 The total magnification of a low power objective lens combined with a of objective lenses for your microscope in the classroom, laboratory,  Be able to calculate the total magnification of your microscope. Total magnification when the objective is in the middle position (or called low power or 10x) is: always begin at 4x and bring your slide into focus using the coarse adjustment knob; move your slide so your specimen is in the middle o Most compound microscopes have objective lenses with magnification of 4X, 10X , and 40X. Ask your instructor for help if you have difficulty focusing your microscope. Resolution Lab Topic 4: Using the Microscope (10 points possible) 1. How to calculate the filed of view when the ocular lens is 40× and the objective lens is 100 The reason for using a microscope is to magnify features to the point where new details can be resolved. What is the magnification under low power? What is the magnification under medium power? What is the magnification under high power? B) Measuring . Each lens has its own magnification power. Have fun with your new microscope. When coupled with the four different objective lenses, the maximum magnification of a microscope can be 1,000X. Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. The HIGH POWER _____ c. The smallest lens is the scanning objective lens. 4. The magnification written on the ocular lens (eyepiece) is _____ The magnification on the Scanning objective _____ Low Power Objective _____ High Power Objective _____ Oct 06, 2008 · You cannot use a ruler under high power because it is too big. m = 400 X. Briefly, NA is related to the capability of your lens to gather lights. O. When using the higher power objective lenses (40x or 100x), only the fin Your microscopes are equipped with several objective lenses, ranging from low to high magnification, including one Total magnification of the specimen is determined by multiplying the magnifying power of the ocular and objective lens Total magnification of the microscope is found by multiplying the power of the determine the diameter of the field of view, place a plastic ruler under the low power (4 x) objective. Apr 07, 2020 · A microscope can have up to four different types of objective lenses. ) 5. 4 Oct 2000 For instance if your ocular is 10x and low power is 4x the total magnification for that lens is 40x. Microscopes that advertise 1600x or anything above 1000x are a waste of time. Follow the path of light through the microscope. Rotate the objective lens part way between the 40x and 100x lens so you can reach the cover slip on your slide. 7. DON’T use the coarse adjustment knob with medium or high power. Compare your result with the rest of the class. Scanning. -to learn how to from the cabinet. . 5X to 75X when combined with 10X ocular lens. Place the microscope slide on the stage (6) and fasten it with the stage clips. While colonies of protozoa can be viewed using 30X on a stereo microscope, other microorganisms such as hydra and daphnia can actually be seen without magnification since they tend to be multicellular . In this lab, you will observe cells using the microscope to determine cell length, shape and arrangement. Step 3: Finally, focus the slide under high power. For example, if through a microscope you can see something 25 times larger than actual size, its magnification Total microscope magnification is determined by multiplying the objective magnification by the eyepiece magnification. The magnifying power of the high-power objective lens is 40X. A. ( It allows you to see objects 4X their size so it is easier to find them on your slide. 5 mm in diameter using a 10X o (Write down your answers and then click at bottom of page for the correct answers. If a microscope has a low power magnification of 100x and a high power magnification of 500x, and a low power field of 1500 μm, what is the high power field in μm? 2. Cover the microscope. If high power is 10X more magnification than the low power, the field of view will be 1/10 as big. power before going to high power. 65 N. ALWAYS leave the microscope with the low power objective in place and the body tube at its highest position. E. What should be done whenever you are finished using a microscope? _____ 11. With two-lens systems, there is a simple method to figure the total magnification when using each objective. The total magnification of an image is the result of the combined •A microscope is a tool that is used to the image under HIGH power •If the eyepiece has a magnification of 10X and you are using the 4x objective lens, what is the total magnification? 10 x 4 = 40 Always carry your microscope EYEPIECE – where you place your ______ and focuses / magnifies the . low-power objective. Magnification is the factor by which an image appears to be enlarged. Use your powers of observation and high and low power objectives to see a variety of cells. d. Bolting silk. To calculate the magnification on a microscope multiply the magnification power of the eyepiece you are using by the objective currently in position. 50 for a 60 X or a 100 X oil immersion lens. o Low power total magnification = _____ o High power total magnification = _____ Microscope Viewing When Resolution is a somewhat subjective value in microscopy because at high magnification, an image may appear unsharp but still be resolved to the maximum ability of the objective. the low power objective is _____x c. 40 Votes) Objective Lenses: Usually you will find 3 or 4 objective lenses on a microscope. 025 for very low magnification objectives (1x to 4x) to as much as 1. The total magnification of the microscope can be calculated by simply multiplying the magnification of the ocular lens by the magnification of the objective being used. This is a small square of fine silk fabric. USING MICROSCOPES **All the steps above must be completed before you attempt to focus on high power** 8. Using two sources of magnification makes this microscope a . Microscope Basics Always use two hands to carry a microscope — one to hold the arm and the other to support the base — and always carry the microscope in an upright position. 6. Here are a few tips for using higher magnification: Make sure the object is in focus- using low power first; Start with the next highest power- depending on the model of your microscope it could be the middle objective lens MagnificationYour microscope has 3 magnifications: Scanning, Low and High. In 1873, Ernst Abbe, working with Carl Zeiss in. Choosing a microscope usually starts with selecting either a Biological or Stereo Microscope depending on your application and the magnification needed. A simple microscope uses only one lens; a magnifying glass could be  17 Apr 2018 Changing the magnification on a microscope also changes the light With an ocular power of 10x, that gives the standard optical For example, if the diameter of your field of view is 1. b) Calculate the high power magnification of this microscope. Remove and clean the slide. m = m₁ × m₂. Resolution. 7. What is the total magnification of your microscope under high power ? If your microscope has a mechanical stage, you will be able to move the slide When coupled with a 10x (most common) eyepiece lens, total magnification is 40x very thin slides and you weren't able to focus on the specimen at hi 20 Jan 2017 Find the total magnification of your microscope. As you increase the Please take time to familiarize yourself with your microscope and its proper use. ) 2. Therefore, the least total magnification of an image is 40x and the highest is 1000x. : Total magnification = Question 10 Calculating the Total Magnification of the Microscope While using the High Power objective lense what is the Total magnification = Question 11 At what total magnification was the ink on the lettere the clearest and most The 100 X objective (1,000X total magnification) requires that a drop of immersion oil be placed between the slide and the lens. View the stained potato on high power. V power you want to find) x original measurement 2. Microscope Calculation Summary. 4. At 1000x magnification you will be able to see 0. Hence, the correct option is (c) "400 X". 78 millimeters under 10x magnif lower power lens the total magnification is: 10X ocular x 10X low power objective = 100X). For most high power applications, a maximum magnification of 400x is sufficient. Therefore, a 10x eyepiece used with a 40X objective lens, will produce a magnification of 400X. Rotate the turret clockwise to the next higher power objective. D. Show your formula and all work. The ocular usually has a magnification of 10X. . 4. Repeat steps 3-5 until you have magnification and focus you want. 000 Total Magnification = Eyepiece Magnification (10x) X Objective Lens Power. 6. (We can only use 2 lenses at a time, not all three. from one side to the other under each power when . Types of Eyepiece: It also determines magnification of a microscope with a digital camera and a camera relay lens. This number is the power of magnification for each of the objective lenses. On high power use the iris diaphragm to improve the resolution. Low. Turn on the power strip and/or microscope. Some microscopes allow you to use oil with the  6 Feb 2015 Magnification is the ability of a microscope to produce an image of an object at a scale larger (or even smaller) than its actual size. Take a clear plastic ruler and examine the millimetre scale under low power. Most educational-quality microscopes have a 10x (10-power magnification) eyepiece and three objectives of 4x, 10x and 40x to provide magnification levels of 40x, 100x and 400x. SURVEY . Multiply ocular magnification times objective magnification. Total magnification = ocular magnification x objective magnification. Name the objective with a power of magnification = 40X. 5. 3 (12pt) T' T. For example, if the ocular lens magnifies 10x and the objective being used magnifies 40x, the total magnification is 400x. Using the microscope 1. A with 25x eyepieces. The use of the magnifying 4. Prepare 2. The microscope should be on a level surface (your desk). 10x. • Avoid prolonged 9 Jan 2018 At high magnification*, the bacterial cells will float in and out of focus, especially if the layer of water between Magnification and its Result The total magnification of the microscope is calculated by multiplyin For example, an eyepiece of x7, used with an objective lens of x10, will give a total magnification of x70. super extra high power lens. Always focus first with the low-power objective. How to Use a Microscope Compound Microscopes. Each objective will have written the magnification. 40x. Prepare your specimen. A multiplied by 1,000 (1000 x 0. When using a 100x objective lens (1000x total power) your working distance might only be 0. What would be the magnification, if you were using a 40X objective? Objective lenses - 4x (scanning), 10x (low power), 40x (high power) Revolving nosepiece; Body tube; Ocular (eyepiece) III. Using the microscope 1. 3. Substage  8 Dec 2020 Basic Microscope Design. Example: Calculate the magnification of a microscope with a 10× ocular lens, a 40× objective lens, and a full-frame digital camera at the oculars and at the camera. 2. High power magnification is 400X. A lens that magnifies ten times is marked 10x. The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide. Low power is 4x, Medium power is 10x, and High power is 40x. A microscope's FOV at any magnification is limited by a number of factors, including the objective lens, the tube-diameter of the microscope's internal optical-system, and the eyepieces. Did this page answer you The magnification of the objective has to be multiplied with the magnification of the eyepiece to give the total Do not use the coarse focus knob with a high power objective, because there is the danger of crashing the objective into Light microscopes use natural light (either ambient light or light from a power source) to magnify the image of small samples or specimens. Examine your microscope. Low Power (______xTM) High-dry Power (_____xTM) C. Example #1. Refer to figure 1 and your microscope to find the structures described on the next Mar 29, 2019 · Then, set the microscope to the lowest level of magnification by turning the nosepiece turret until the shortest lens is above your sample. The eyepiece lens is a 10 times magnification, or 10X. What should be done whenever you are finished using a microscope? _____ 11. Objective Place a microscope slide with your specimen on the stage under the 11. “X” is placed after obtaining the total The standard microscope is that the eyepiece is 10x magnification, and three types of powered magnification helps it magnify even more. ) b) If the diameter of the low power field is 2 mm, what is the diameter of the high power field of view in mm ?. 14. The magnification of a light microscope is formed using a mixture of the powers of the eyepiece and the objective lens. 300 seconds . The total magnification of 40 means that the object appears forty times larger than the actual object. Position your microscope with the arm towards your chest one hand length from the edge. This is one reason why you should start looking at your specimen using lower power to learn your way around a new specimen before you move sequentially to higher power (always start at 4X). Magnification is the factor by which an image appears to be enlarged. Only use the fine adjustment knob (small) to bring object into focus. 65 N. Apr 19, 2009 · The SCANNING POWER objective is the smallest objective lens and has the lowest magnification, probably 4x on your 'scope. Ocular lens. You may also need to recenter. If the microscope has a fourth objective lens, the magnification will most likely be 100x. Show your formula and all work. total magnification of an image is the result of the combined magnifications working distance; that is, low power objectives have longer working distances than The magnification of an object is determined by the power of the ocular and For example, a 10X ocular used with a 4X objective yields a total magn You can determine the magnifying power of the combination of the two lenses by The diameter of the field of view under high power can be calculated using if you determine that your field of view is 2. View Page Off-Line: Right Click On PDF Icon To Save Target File To Your Computer. What will be the field of view if we change the objective lens to 100×? Dec 13, 2017 · Magnifying power on the eyepiece, m₂ = 10 X, We have to find the total magnification. Microscope- Focusing, Total Magnification DRAFT. the and a 10× low power objective lens has a low power total magnification of 100×. The rendering of detail; high magnification without good &nb The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. When looking through your microscope Microscope magnification is the microscope’s ability to enlarge an image of an object through a series of lenses to a size multiple times larger than the actual size of the object. Following light through the microscope. It takes practice! The good news is that you don't need high power that often. ) Why do oil immersion objectives have higher numerical apertures than dry (non-oil) lenses? They are If the total magnification of a microscope is 200 tuning the focus on low power, and focusing the image on medium and high power. Feb 28, 2020 · 4. What might be the problem with this microscope? 3. It will be a whole number greater than 1 and is usually followed by an “x”, as in 10x magnification. e . 7. While the origins are While your specimen is in focus on high power, move the condenser aperture lever from left to right . The total magnification of a typical compound microscope with 10x eyepiece and 4x, 10x, 40x, 100x objectives will be 40x, 100x, 400x and 1000x depending on the lenses used. the magnifying power for that lens. It is used to view an object in even greater details. ocular (eyepiece) lens 10X and high power objective lens 20% Microscope Parts & Their Functions. What is the total magnification if the microscope is on low power (20X)? _____ 12. At 400x magnification you will be able to see 0. LOW power objective. Problem #4: When I moved to a higher power, everything disappeared! Return to the previous (lower power) objective. This step is done with the naked eye May 17, 2016 · You can use a microscope to look very closely at things or to magnify things that are very small. The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes. To find the total magnification of a microscope, just multiply the two magnifications. Total. It holds the objective lenses. S. The numbers printed on side of each objective as “____ X” to indicate the magnifying power of the lens. Oculars are normally 10X or 12X. ( 6 Jul 2018 For optical instruments in general, resolution or resolving power is the ability to see fine details in an image. Name the objective with a power of magnification = 10X. (For example, 4 X times 10 X = 40 X TM). Identify two main differences between a compound microscope and a dissecting microscope. 1. Return all materials to their proper place. Show your formula and all work. the item should be Placed between the eyepiece tube and the eyepiece. ) Draw what you see below. Use the coarse focus knob to readjust focus. o Low power total magnification = _____ o High power total magnification = _____ Microscope Viewing When Total Magnification Estimate the number of starch grains that you can see. If the eyepiece has a magnification of 10X, what is the total magnification with a 10X objective? _____ What is the total magnification with a 45X objective? _____ 2. power: use a . 3 mm would be Therefore, the total magnification of an object viewed under low power is 100 . Even scientists use low and medium powers for much of their work. ocular (eyepiece) lens 5X and low power objective lens 15X. Magnification The microscope magnifies (increases apparent size) very small objects. Multiply scanning-power field of view to calculate the diameter of the -power lens by the power of the high-power lens. There are about 1- 4 objective lenses placed on one microscope, in that some are rare facing and others face forward. The total magnification will be 7. B. Objective Lenses- Magnify the. With an ocular power of 10x, that gives the standard optical microscope a range of overall magnification from 40x to 1000x. The lens will be extremely close to the specimen! The working distance and the amount of vertical motion of the microscope will also affect the maximum specimen height. ). M HP is the higher power objective lens magnification, and. The magnifying power of a microscope is an expression of the number of times the object being examined appears to be enlarged and is a dimensionless ratio. Each lens has its own magnification power. Sample problem: If the ocular is 10X and the objective is 43X, the total -to determine the total magnification of the microscope -to describe changes in the field of view and light when going from low to high power using the compound light microscope and how it affects what you actually see as you observe it-to learn how to properly focus when going from low power to high power Materials: Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x). 2. Sep 16, 2018 · Observing objects under high power can be tricky. 6 Jul 2018 Moreover, there is typically a lower and higher limit of magnification which define As an example, a microscope with a 40× objective (M O = 40×), total tube For optical instruments in general, resolution or resolvin This lab is designed as an introduction to microscope use and microscopy for This activity should be done using the low power objective. 10x. a. 8. Go to the higher power objective and use only the fine focus. Question 9 Calculating the Total Magnification of the Microscope What is the Total Magnification when using the Low Power ojective lence. 6. Record the number of your microscope here: (found on the arm of the microscope). Parts of Place your slide in the slide holder on the stage with the beam of light passing through your specimen. Record the Magnification of all power levels for your microscope in table 1. 6 for high-performance objectives utilizing specialized immersion oils. Example: Calculate the magnification of a microscope with a 10× ocular lens, a 40× objective lens, and a full-frame digital camera at the oculars and at the camera. In three steps using complete sentences, describe how to make a proper wet mount of the letter e. Low power. ) May 19, 2020 · Numerical Aperture (NA) determines the limit of the Resolution that your microscope can achieve. 1 mm, what is the minimum magnification needed to make an object measuring 1 um visible? 10x 100x 1,000x 10,000x 100,000x If the total magnification of a microscope is 2000X with the use of a 10X ocular lens, what is the magnification of the objective lens? 10X 20X 200X 2000X 20,000X Nov 19, 2010 · To find the total magnification of your microscope as you are using it, multiply the ocular lens power times the power of the objective lens that you are using. Others also have a 100x magnification power. Place the centre of one of the scale marks along the edge of the field as shown Each objective lens has a different magnification of power written on it (such as 4, 10, 40, or 100). List the magnification for each objective on your microscope below. 9. ALWAYS leave the microscope with the low power objective in place and the body tube at its highest position. a. Define field of view and describe how the field of view changes with Low Power High Power (dry) Oil Immersion TOTAL MAGNIFICATION 1. Nose piece – also known as the revolving turret. Increase the power of magnification until you are observing the subject at high power (400X). CALCULATE the total magnification that may be achieved with each of the Once you have it focused and centred on medium power, you can progress to high power. The total magnification is the eyepiece lens x objective lens!! Make sure your paramecium is in the center of your low power field of view before you switch to the high power lens. Example: Low power: Objective lens = 10X Ocular lens = 10X Total magnification at Low Power = 10X(10X) = 100X 3. Tags: Question 4 . LOW POWER is the next largest lens, probably with a magnification of 10x. 2. (1 point) 2. c. high-power objective. What happens to the "e". Examination and dissection of the parts of particular interest are carried out under higher power magnification. The total magnification for this lens is equal to 400x magnification (10x eyepiece lens x the 40x objective equals 400). 1. Magnification: the process of enlarging the size of an object, as an optical image. as the field of view. Find the total magnification for each objective lens by multiplying the power of the eyepiece by the In calculating the size of the high-magnification field of view, B – Fine adjustment: to focus specimen under high power possible magnification that can be obtained when using this microscope? Support your answer. adjustment knob, the subject can be focused at this higher magnification. Magnification of 400x is the minimum needed for Calculating total magnification Practice problem: Calculate the low-power and the high-power total magnifications of a microscope with an eyepiece magnification of 10x, a low power objective of 20x, and a high-power objective of 40x. Oil immersion Make sure your workstation is set up ergonomically to use the microscope. 1. The total magnification is determined by multiplying the magnification High Power, 40x, 10x, 400x. For example, if the ocular lens was 15X and the objective being used was 10X, the total magnification being used is 15 x 10 = 150X. To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X. Total Magnification-how much the image is magnified in the microscope-calculated for each objective power. What is the total magnification of an item viewed with the: 3. The largest lens is the HIGH POWER objective. Eyepiece is located at the top of the microscope. Recenter the specimen, adjust the diaphragm, and adjust the position of the condenser to increase the contrast of the specimen. The eyepieces are typically the determining factor, which is why they should be marked with a field-number (FN), which represents the diameter of the field Most light microscopes have four objective lenses: the scanning objective lens (4x), the low-power objective lens (10x), the high-power objective lens (40x), and the oil immersion objective lens enlarging the image more just before it reaches your eye. 9 Fine adjustment knob To move the body tube slowly to allow focussing under High Power magnification. On your worksheet below calculate the total magnification for each ocular/objective combination on your microscope. Therefore we can perform a simple calculation to determine the FOV under high power: High power magnification = Low power FOV Low power magnification High power FOV 400X = 4. 2. May 15, 2020 · Total Magnification power = Magnification of the objective lens x Magnification of the eyepiece. magnification. The power of magnification is marked by a number with an x next to it. A microscope has a 20 X ocular (eyepiece) and two objectives of 10 X and 43 X respectively: a. The compound microscope uses a variety of lenses to magnify objects up to 1000 times. switch to higher power. Prepare a wet mount slide of a lowercase letter “e” title font. 5x and the stereo microscope has a 1. Optical microscopes are the oldest design of m choose to have your students complete this Master while viewing the program or to The total magnification of the microscope is computed by multiplying the want to increase the magnification by switching to a higher power objective The compound light microscope is by far the most utilized piece of equipment in the biologist's tool chest. The eyepiece produces a power of 10x and the objective lens can produce various different powers, so if it were to produce a power of 100x , the final magnification would be 1000x (10 x 100). Show your formula and all work. b. 2. The magnification power of an Auxiliary lens will be multiplied with the total magnification power of a stereomicroscope. 27. In addition to this, the ocular lens (eyepiece) has a magnification. Using the Microscope - Lab Tech which objective is high power? 40x What is the total magnification achieved with a 10x ocular and a 40x objective? Question: QUESTIONS What Is The Total Magnification Of Your Microscope When Using The Oil Immersion Objective? Arial T TTTT Paragraph % DOO S. The value of NA ranges from 0. Therefore, the total magnification is 40x. Look at the objective lens (3) and the stage from the side and turn the focus knob (4) so the stage moves upward. resolving power – its numerical aperture or NA. NEVER use the coarse adjustment when focusing on the medium and high power objectives! 16 Jul 2019 Fungi are larger than bacteria, and their single-cell stage, called yeast, can be viewed with a light microscope. 7. Parasites are Resolution or Resolving Power The ability to distinguish fine detail with a magnifying ins oculars are engraved with their magnifying powers; oculars magnify. 1. Cover the microscope. Problem #5: The image is blurry on all powers. The microscope should be on a level surface (your desk). To find the total magnification of a microscope, multiply the magnification of the eyepiece (usually 10x) by the magnification of the objective lens. The magnification of each lens is: Scanning objective: 4X Low power objective: 10X High power: 40X To calculate the microscope’s total magnification using any given lens, you use the following formula: Total Magnification = Using a light microscope. What is the total magnification of your microscope under low power? (Use the numbers printed on your low-power objective and eyepiece if present. The total magnification is the ocular x objective Low power magnification High Power field diameter Low power field diameter High power magnification = 10 = X 50 X = 20 mm 50 2. Most of the things you are going to study with a microscope look better at low and medium power, anyway. Created by Mahesh Shenoy. Auxiliary lens used to alter the total magnification power of Dissecting microscope. It also determines magnification of a microscope with a digital camera and a camera relay lens. High-power objective: If your microscope has three objective lenses, this lens will be the longest. Low-, medium-, and high-power lenses that further magnify the Always carry the microscope by holding the arm of the microscope with one hand and magnifies the image 10X, the total magnification of the object unde Compare your microscope with the figure on the following page and identify the High Power. Let's solve a numerical on compound microscope. About. Define field of view and describe how the field of view changes with 3. M LP is the lower power objective lens magnification. 10x. What is the total magnification of your microscope when using high power? Part II. Without knowing the brand or different powers. The total magnification for each objective is calculated by multiplying the magnification of the ocular and objective lens on your microscope. A microscope has a 20 X ocular (eyepiece) and two objectives of 10 X and 43 X respectively. What is the total magnification of an item viewed with the: The PLAN objective _____ The LOW power objective _____ The HIGH POWER _____ 4. Complete the data table on your answer sheet to find the total magnifications of each objective on our microscopes. The total magnification is determined by multiplying the power of the objective by the power of the ocular. What would be the magnification, if you were using a 40X objective? 1) Lowest Magnification 2) Low Magnification 3) High Magnification 4) Highest Magnification In image 1, we can see a model of DNA on a table with a water bottle and a large area of the room. 2. Example #2 The total magnification of any specimen is equal to the power of the ocular lens multiplied by the power of the objective lens used. Total magnification = (60X)(20X) Total magnification = 1200X Data Table 1: Our NF Microscope Magnification Objective Magnification of Objective Lens Scanning Power Low Power High Power Part B: 4. Place the specimen on the The ocular, located at the end of the body tube, has a magnification power of 10X. In addition to this, the ocular lens (eyepiece) has a magnification. Explain why the specimen must be centered in the field of view on low power before going to high power. Example: Low power: Objective lens = 10X Ocular lens = 10X Total magnification at Low Power = 10X(10X) = 100X 3. On your microscope, it probably has a magnifying power of 40x or 43x. view): the . 1. Some stereo microscopes equipped with continues zoom objective lens with magnification from 0. Observe how much of the paramecium you can see now. Based on the maximum resolving power, also a useful range of viewing distance, i. 6. It is usually expressed in the form 10× (for an image magnified 10-fold), sometimes wrongly spoken as “ten eks”—as though the × were an algebraic symbol—rather than the correct Insert a micrograph of the stained epithelial cheek cell under LOW power and HIGH power. For example, if the ocular lens of a microscope has a power of 5x and you use an objective that is 10x, then the total magnification of the microscope at that time is 50x (5x10=50). Mashups. Center the object in the field of view. 1. Eyepiece Magnificati Magnification. Upon completion, turn the nosepiece to the lowest power objective. 4. 5/5 (589 Views . Write The illuminated specimen is viewed through 2 magnifying lenses; 1) the magnifying lens of the objective located on the rotating nose-piece which is Each objective is clearly marked indicating its magnification power. 5. The total magnification reaching your eye is the product of the magnifications of ocular and objective. Low power magnification × Low Power field of view = high power magnification × high power field of view Microscope magnification calculation examples: When the ocular lens is 10× and the objective lens is 10×, the field of view is 800 µm. 5x magnification. To determine total magnification while viewing a specimen, simply multiply the magnification of the eyepiece by the magnification of the objective that is in position: total magnification = magnification of eyepiece x magnification of objective For example, if the eyepiece is 10x and the scanning power objective is 5x, then the total magnification under low power is 10 x 5 = 50x. DON’T use the coarse adjustment knob with medium or high power. 10x. Always focus first with the low-power objective. At high power, you should ONLY use the fine adjustment knob to bring your specimen back into focus. The total magnification of an image is the result of the combined magnifications of the 1) eyepiece and diaphragm 2) objective and eyepiece 3) objective and mirror 4) low-power and high-power objectives The total magnification possible with a standard compound microscope is the multiple of the magnification factor of the eye piece lens and the objective lens in use. If the eyepiece magnification of a microscope is 10x and the objective lens in use has a magnification of 4x, calculate the magnification of the microscope. The amount of light transmitted to your eye is greatest at the low power. 4 mm 50 . (1 points) 3. In order to ascertain the total magnification when viewing an image with a compound light microscope, take the power of the objective lens which is at 4x, 10x or 40x and multiply it by the power of the eyepiece which is typically 10x. Briefly explain how to determine the total magnification when using a compound light microscope. 5 = 37. 33 mm. a. scanning objective. Use only enough magnification as needed. 1. _____ b. Preparing and using a Wet Mount 8. It measures at 4X, and it is the shortest lens of the four. HIGH-POWER OBJECTIVE – a large lens with_________ magnifying power (______ lens) . C. 3. 5mm 40X x 4 4 1 2 4 3 Jun 29, 2019 · Magnification: Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x). 5X. Total Magnification Estimate the number of starch grains that you can see. 8 mm The ratio of low to high power is 20/50. through the microscope. (1 point) 2. With your scissors cut out the letter "e" Although higher magnifications will allow you to see smaller objects, being able a dissecting microscope from the cabinet and bring it back to your lab table. Using 40X total magnification (low power) while looking at the letter"e", move the stage forward and backward and right then left. Identify two main differences between a compound microscope and a dissecting microscope. If you lose the image, go back to the low power lens, focus it, put it in the center, switch to the high power lens, and use fine focus only. Finally, look through the eyepiece and focus the microscope by using the coarse focus and fine focus knobs. Make your estimate by doing the following calcu other words, if an object is not centered in the field of view under low magnification, it may not power (by turning the knob on the side of the microscope), record the diameter of the Before returning your dissecting microscope What is the total magnification of the examples below? exa. High power objective:40x. 3x auxiliary lens on the microscope. Lab: Using a Microscope. When using a light microscope it’s important to start with the low power objective lens. Resolution 9. Microscopes use lenses to magnify objects. Total magnification of an image is given by the product of microscope's objective and magnifying power of the eyepiece i. 10. The medium size objective is the LOW POWER OBJECTIVE. Total magnification. b) Calculate the high power magnification of this Sep 23, 2020 · Use the lowest magnification. 400x. multiplying the power of the objective lens times the power of the ocular lens. The field of view under high power will be 2 mm X 1/10 = 0. In order to ascertain the total magnification when viewing an image with a compound light microscope, take the power of the objective lens which is at 4x, 10x or 40x and multiply it by the power of the eyepiece which is typically 10x. Did the results support your hypothesis (explain)? Use 2-4 complete sentences to answer these questions: Obtain your microscope from your teacher. Thus the magnification of a microscope is: magnification power of the eye-piece multiplied by the power of the objective lens. To determine the field of view under high power, use this following formula: (Low power diameter) x (Low power magnification) = (High power diameter) x (High power magnification) Using the eyepiece reticle to measure the size of objects: Your microscope also has a reticle in one of the eyepieces. Briefly explain how to determine the total magnification when using a compound light microscope. Familiarize yourself the low power objective is ______x the high power objective is ______x. To find the total magnification of your microscope as you are using it, multiply the ocular lens power times the power of the objective lens that you are using. 45mm, or 450 microns. The closer the object is to the eye, the larger the angle that it subtends at the eye, and thus the larger the object appears. Oil immersion objective (on microscopes with four objective lenses): Usually it has a 100X magnification. Total Magnification: To figure the total magnification of an image that you are viewing through the microscope is really quite simple. lens the total magnification is: 10X ocular x 10X low power objective = 100X). Higher values of _____ What Is The Total Magnification Of Your Microscope Under High Power? ( Use The Numbers Printed On Your High-power Objective And Eyepiece. Examine it with low power, and then raise the magnification. There should 18 Jul 2016 The total magnification of a specimen viewed under a compound light microscope is determined by multiplying C. 3. B. 1pc Biological Microscope Use 2X Microscope Barlow Eyepiece Magnification lens. high power? _____ Bonus Lab: Determining the “Field of View” Procedures: a. high power 40X or 43X or 45X . m = 40 X × 10 X. For instance, if your eyepiece reads 10X/22, and the magnification of your On high power, you will need to maximize the brightness of the light source and regulate the iris diaphragm. For example, you can achieve 1,000x magnification by using a 40x/0. For example, if the ocular lens of a microscope has a power of 5x and you use an objective that is 10x, then the total magnification of the microscope at that time is 50x (5x10=50). 40 or 1. the microscope by its arm and the other hand should be place under the base of the microscope to adjustment knob moves the stage a large visible distance with a single turn and as such should What would be the total magnifying po At low magnification the working distance is relatively long. The common ocular magnifies ten times, marked as 10x. Q. Using low-power objective lens, ge Most microscopes have on their base an apparatus called a condenser, which condenses light rays to a strong beam. 4. The benefit of a high-power objective lens is that it provides the most magnification possible with most standard, monocular (single) eyepiece microscopes. Low. 9. Each objective lens will have written the magnification. 2 mm =200um # of cells 10 cells Apr 08, 2020 · If the magnification power of the ocular lens is 10x and that of the objective lens is 4x, total magnification is 40x. Click the high power objective into place, it should be very close to the stage. HTML, CSS Path:p QUESTION 6 What Is The Total Magnification Of Your Microscope When Using The High Dry Objective? Microscopes magnify an image by use of lens found in the eye-piece, which is also known as the ocular lens. • Be able to explain stage; never use the coarse focus on high power! Condenser. 180mm, or 180 microns. a microscope has a power of 5x and you use an objective that is 10x, then the total magnification of the microscope at that time is 50x (5x10=50). Each objective will have written the magnification. 1. 5 x 1. Return your dissecting microscope to the cabinet. Microscope is an optical instrument that uses lens or combination of lens to produce a magnified images that are too The total magnification the product of the magnifications of two lens systems. What total magnifications are possible with your compound microscope? Low power:_____ medium power:_____ high power:_____ 2. Total magnification is also dependent upon the tube length of the microscope. This looks like a small If the minimum image size the human eye can detect is 0. 4. Rinse and dry your slide. Adjust the magnificati -to demonstrate the proper procedures used in correctly using the compound light microscope. A student sees the image to the left when observing the letter "f" with the low-power objective lens of a microscope. Examine the wheel on the left side of the base. Therefore, a 10x eyepiece used with a 40X objective lens, will produce a magnification of 400X. The FOV gets smaller as you move to a higher magnification. 1. To get the total magnification level, multiply the magnification of the objective used (ex: 10X eyepiece * 40X objective = 400X total magnification). Make a drawing below. The total magnification using the lenses can be determined by multiplying the objective lens with the ocular lens. Abbe and Numerical Aperture. Oil Immersion. The image is further magnified by the objective lens. You will have to multiply the eyepiece magnification by the objective magnification to find the total magnification before dividing the field number. e not too high nor too low, and therefore depending on the 3. Email  They can be visualized by electron microscopes and their measurements are in nm (nanometer). Ensure your specimen is centered in your viewport. You will have to multiply the eyepiece magnification by the objective magnification to find the total magnification before dividing the field number. For instance, if your eyepiece reads 10X/22, and the magnification of your Microscope - Microscope - Magnification: It is instinctive, when one wishes to examine the details of an object, to bring it as near as possible to the eye. Magnification. Switch to the high-power objective (43x) and adjust the focus using the fine adjustment knob. Click to see full answer. Function :Doubling Total Magnification power (such as ,your microscope total magnification is 400X ,you use it, the magnification will increase 800X ). 1. the high power objective is _____x. If the eyepiece of your scope has a power of 10, and the selected objective lens has a power of 20, what is the total magnification? answer choices 20x For instance, using a 5X objective with a 10X eyepiece yields a total visual magnification of 50X and likewise, at the top end of the scale, using a 100X objective with a 30X eyepiece gives a visual magnification of 3000X. 4. A compound microscope is a microscope that uses multiple lenses to enlarge the image of a sample. Objectives are typically as follows: a. 2. Table 4 Total magnification data, MTOT VIS and M DIS (Equations 2 and 4b), for a digital microscope with 5 Although a magnifying glass technically qualifies as a simple light microscope, today's high-power—or compound— microscopes To get the total magnification level, multiply the magnification of the objective used (ex: 10X eyepiece The total magnification at which any specimen is being viewed is the product of the ocular lens magnification and the magnification of the objective lens in use. 40x. 4x) is clicked into position. Jan 25, 2021 · They have a magnification power of 40x-100X. Using the terms learned above, complete the following list… For example, a high-power microscope with 1,000X magnification doesn’t work well when looking at coin, because the magnification would be be too great to see any details of the coin. Name the objective with a power of magnification = 4X. The standard objective lenses magnify 4x, 10x and 40x. Magnification. The average constant for my microscope at home is 145. Nose piece – also known as the revolving turret. For example, for a microscope with a 10× eyepiece and 45× objective, the magnification is 10 × 45 = 450 and the field of view is 0. Multiply scanning-power field of view to calculate the diameter of the equation for finding F. Mar 03, 2021 · Lab Topic 4: Using the Microscope (10 points possible) 1. Focus the microscope using the coarse focus knob. For example: if the magnification of the objective is 45x and that of the eyepiece is 10x, the total magnification of the specimen will be 450x. What is the field diameter for my microscope at high power? Field diameter × total magnification = a constant Field diameter × 400X= 145 Field diameter = 145/400 Field diameter = 0. There are about 1- 4 objective lenses placed on one microscope, in that some are rare facing and others face forward. These microscopes will not have good resolution, and there is no sample that needs to be viewed above 1000x. What is the total magnification if the microscope is on low power. 11. 1. Numerical aperture determines the resolving power of an objective, but the total resolution of a microscope system is also dependent upon the numerical aperture of the If your microscope uses both an eyepiece and an objective lens, there is one step to do before you find the FOV. It has a magnification power of 10X. power (by turning the knob on the side of the microscope), record the d Acquire skill in using the light microscope by carefully following all directions. However, the total magnification of 1,000x exceeds the value of 0. If an object is brought too close, however, the eye can no longer form a clear image. Apr 21, 2017 · b. what is the total magnification of your microscope when using high power 